Peptide Reconstitution Calculator
Calculate exact mixing ratios, syringe units, and dosages for any peptide. Free, instant, and easy to use.
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Dose Reference Table
| Dose (mcg) | Volume (mL) | Units | Doses / Vial |
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How to Reconstitute Peptides
Follow these steps for safe and proper peptide reconstitution:
- Gather supplies: Lyophilized peptide vial, bacteriostatic water (BAC water), alcohol swabs, and an insulin syringe.
- Clean both vial tops with alcohol swabs and let them air dry for 30 seconds.
- Draw bacteriostatic water into your syringe - use the calculator above to determine the right amount.
- Inject water slowly into the peptide vial. Aim the stream against the glass wall, not directly onto the powder cake.
- Gently swirl the vial until the powder is fully dissolved. Never shake - this can damage the peptide chains.
- Store in the refrigerator at 2-8 degrees C (36-46 degrees F). Reconstituted peptides with BAC water typically last 3-4 weeks.
Understanding Insulin Syringe Units
Insulin syringes measure volume in "units." On a standard U-100 syringe:
- 100 units = 1.0 mL
- 50 units = 0.5 mL
- 10 units = 0.1 mL
- 1 unit = 0.01 mL
Syringes come in three common sizes:
- 0.3 mL (30 unit) - Best for small doses. Tick marks every 0.5 units for maximum precision.
- 0.5 mL (50 unit) - Good all-around size. Tick marks every 1 unit.
- 1.0 mL (100 unit) - For larger volume injections. Tick marks every 2 units.
Tip: Always use the smallest syringe that can hold your dose. Smaller syringes have finer graduations, making it easier to measure accurately.
Frequently Asked Questions
Gather your lyophilized peptide vial, bacteriostatic water, alcohol swabs, and an insulin syringe. Swab both vial tops with alcohol. Draw your desired amount of bacteriostatic water into the syringe. Inject the water slowly into the peptide vial, aiming the stream against the glass wall - not directly onto the powder. Gently swirl (never shake) until fully dissolved. Store reconstituted peptide in the refrigerator.
The amount depends on your desired concentration. Common amounts are 1mL or 2mL. Adding less water creates a more concentrated solution (fewer units per dose), while adding more water makes it easier to measure small doses accurately. Use the calculator above to find the right amount for your specific peptide and dose.
On a standard U-100 insulin syringe, 100 units equals 1mL. So 10 units = 0.1mL, 50 units = 0.5mL, and so on. Insulin syringes come in three common sizes: 0.3mL (30 units), 0.5mL (50 units), and 1.0mL (100 units).
Reconstituted peptides stored in the refrigerator (2-8 degrees C / 36-46 degrees F) typically remain stable for 3-4 weeks when mixed with bacteriostatic water. The benzyl alcohol preservative helps prevent bacterial growth. Always check for cloudiness or particles before use - discard if the solution is not clear.
Bacteriostatic water contains 0.9% benzyl alcohol as a preservative, which inhibits bacterial growth and allows multiple withdrawals over weeks. Sterile water has no preservative and is for single-use only. For peptides used over multiple doses, bacteriostatic water is strongly recommended.
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